1716/2011 - Avaliação in situ da genotoxicidade de triazinas utilizando o bioensaio Trad-SHM de Tradescantia clone 4430 In situ genotoxity evaluation of triazines using Tradescantia clone 4430 Trad-SHM bioassay
• Carina Patussi - Patussi, C. - Joaçaba, SC - Universidade do Oeste de Santa Catarina (UNOESC), Joaçaba, Santa Catarina, Brasil - <caripatussi@yahoo.com.br>
Coautor(es):
• Márcia Bündchen - Bündchen, M. - Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Sul (IFRS), Porto Alegre, Rio Grande do Sul, Brasil. - <marcia.bundchen@poa.ifrs.edu.br>
Área Temática:
Saúde e Ambiente
Resumo:
O bioensaio da mutação do pelo estaminal de Tradescantia clone 4430 (Trad-SHM) foi utilizado para avaliar a genotoxicidade de um herbicida composto por triazinas (atrazina e simazina) após exposição in situ. Trinta vasos da planta foram expostos durante a aplicação do herbicida (grupo teste) mantendo-se um grupo controle em casa de vegetação. A genotoxicidade foi expressa em termos de eventos mutantes pink (EMPs) e a análise dos dados foi realizada através do teste t de Student em oito dias de avaliação (C8D = controle 8 dias; T8D = teste 8 dias) e no dia de pico (CPD = controle dia de pico; TPD = teste dia de pico). A exposição ao herbicida causou um número significativamente maior de EMPs no grupo teste (T8D = 2,27; TPD = 4,69) do que no controle (C8D = 0,71; CPD = 0,62), demonstrando a existência de risco genotóxico associado ao uso das triazinas, sendo o bioensaio Trad-SHM uma eficiente ferramenta para avaliar o potencial genotóxico destes contaminantes ambientais causadores de efeitos adversos à saúde humana.
Palavras-chave:
pesticidas
genotoxicidade
mutações pink
meio ambiente
saúde humana
Abstract:
Tradescantia 4430 clone stamen hair mutation (Trad-SHM) bioassay was used to evaluate the genotoxicity of a herbicide comprising triazines (atrazine and simazine) by in situ exposure. Thirty plant vessels were exposed during the herbicide application (test group) and a control was maintained in a greenhouse (control group). Genotoxicity was expressed in terms of pink mutation events (EMPs) and the data analysis was performed by Student‘s t test comparing control and contaminated group after eight-days (C8D = 8 days control; T8D = 8 days test) and peak day (CPD = peak day control; TPD = peak day test). The exposure to the herbicide caused a significantly higher number of EMPs in the contaminated group (T8D = 2.27; TPD = 4.69) that of the control (C8D = 0.71; CPD = 0.62). This demonstrates that the Trad-SHM bioassay is sensitive and efficient and can be used as tool to assess the genotoxic potencial of environmental contaminants like triazines associated with adverse effects on human health.
Keywords:
pesticides
genotoxity
pink mutations
environment
human health
In situ genotoxity evaluation of triazines using Tradescantia clone 4430 Trad-SHM bioassay
Resumo (abstract):
Tradescantia 4430 clone stamen hair mutation (Trad-SHM) bioassay was used to evaluate the genotoxicity of a herbicide comprising triazines (atrazine and simazine) by in situ exposure. Thirty plant vessels were exposed during the herbicide application (test group) and a control was maintained in a greenhouse (control group). Genotoxicity was expressed in terms of pink mutation events (EMPs) and the data analysis was performed by Student‘s t test comparing control and contaminated group after eight-days (C8D = 8 days control; T8D = 8 days test) and peak day (CPD = peak day control; TPD = peak day test). The exposure to the herbicide caused a significantly higher number of EMPs in the contaminated group (T8D = 2.27; TPD = 4.69) that of the control (C8D = 0.71; CPD = 0.62). This demonstrates that the Trad-SHM bioassay is sensitive and efficient and can be used as tool to assess the genotoxic potencial of environmental contaminants like triazines associated with adverse effects on human health.
Palavras-chave (keywords):
pesticides
genotoxity
pink mutations
environment
human health
Patussi, C., Bündchen, M.. Avaliação in situ da genotoxicidade de triazinas utilizando o bioensaio Trad-SHM de Tradescantia clone 4430. Cien Saude Colet [periódico na internet] (2012/mar). [Citado em 22/12/2024].
Está disponível em: http://cienciaesaudecoletiva.com.br/artigos/avaliacao-in-situ-da-genotoxicidade-de-triazinas-utilizando-o-bioensaio-tradshm-de-tradescantia-clone-4430/9548?id=9548&id=9548