0243/2015 - Caracterização de perfis enzimáticos de cepas do Aedes aegypti do Estado do Rio Grande do Norte, Brasil Characterization of Enzymatic profiles of Aedes aegypti strains from the State of Rio Grande do Norte, Brazil
• Maria Jocileide de Medeiros Marinho - MARINHO, M.J.M - UERN, DCB - <jocileidemm@gmail.com>
• Richardeson Fagner de Oliveira Grangeiro - Grangeiro, Richardeson Fagner de Oliveira - UERN, DCB - <richardesonfagner@hotmail.com>
• Wogelsanger Oliveira Pereira - Oliveira Pereira, Wogelsanger - Universidade do Estado do Rio Grande do Norte, Ciências Biomédicas Mossoró, RN, 59611070, BR - <wogel.uern@gmail.com>
Área Temática:
Saúde e Ambiente
Resumo:
Este estudo foi realizado em quatro cepas de mosquitos Aedes aegypti da cidade de Mossoró, Rio Grande do Norte, objetivando avaliar os perfis de atividade enzimática e correlacioná-los com os mecanismos de resistência a inseticidas. Foram quantificadas as seguintes enzimas de detoxificação: Oxidase de Função Mista (MFO), PNPA-esterase (PNPA-EST) e acetilcolinesterase (AChE). Os perfis das populações foram comparados com os perfis da cepa Rockefeller através do teste de Kruskal-Wallis e do teste de comparações múltiplas de Dunn (p <0,05). O percentil 99 dos valores de atividade enzimática da cepa referência foi calculado para cada enzima, e o percentual de indivíduos acima do percentil 99 foi quantificado. As atividades enzimáticas foram classificadas como "Inalterada" (<15%), "Alteração Identificada" (> 15% e <50%), e "Substancialmente Alterada" (> 50%). A análise estatística revelou diferenças significativas nos perfis de MFO e AChE, que são fundamentais na determinação de perfis de resistência a inseticidas. Os níveis alterados de atividade enzimática demonstram que essa alteração pode desempenhar um importante papel na resistência a inseticidas. Portanto, é de fundamental importância que o combate aos vetores seja concomitante ao monitoramento dos perfis enzimáticos das populações.
Palavras-chave:
Aedes aegypti
Dengue
Mecanismos de resistência
Inseticidas
Abstract:
This study was conducted in four strains of Aedes aegypti mosquitoes to evaluate the enzymatic activity profiles in the city of Mossoró, Rio Grande do Norte, and correlate them with biochemical mechanisms of resistance to insecticides. Mosquitos were used to quantify the following detoxification enzymes: Mixed-Function Oxidase (MFO), PNPA-esterase (PNPA-EST), and Acetylcholinesterase (AChE). The profiles were compared statistically with profiles from the Rockefeller strain, through the Kruskal-Wallis test and Dunn‘s multiple comparisons (p<0.05). The 99 percentile of the values of enzyme activity from the reference strain was calculated for each enzyme, and the percentage of individuals above the 99 percentile was quantified. The enzyme activities were classified as "Unchanged" (<15%), "Identified change" (>15% and <50%), and "Substantially changed" (>50%). The statistical analysis revealed significant differences in the MFO and AChE profiles, which are fundamental in the determination of profiles of resistance to insecticides. The altered enzymatic activity showed that the changes could have an important role in exposing resistance to insecticides. Therefore, monitoring enzymatic profiles in mosquito populations concomitantly to combating vectors is of fundamental importance.
Keywords:
Aedes aegypti
Dengue Fever
Resistance mechanisms
Insecticides
Characterization of Enzymatic profiles of Aedes aegypti strains from the State of Rio Grande do Norte, Brazil
Resumo (abstract):
This study was conducted in four strains of Aedes aegypti mosquitoes to evaluate the enzymatic activity profiles in the city of Mossoró, Rio Grande do Norte, and correlate them with biochemical mechanisms of resistance to insecticides. Mosquitos were used to quantify the following detoxification enzymes: Mixed-Function Oxidase (MFO), PNPA-esterase (PNPA-EST), and Acetylcholinesterase (AChE). The profiles were compared statistically with profiles from the Rockefeller strain, through the Kruskal-Wallis test and Dunn‘s multiple comparisons (p<0.05). The 99 percentile of the values of enzyme activity from the reference strain was calculated for each enzyme, and the percentage of individuals above the 99 percentile was quantified. The enzyme activities were classified as "Unchanged" (<15%), "Identified change" (>15% and <50%), and "Substantially changed" (>50%). The statistical analysis revealed significant differences in the MFO and AChE profiles, which are fundamental in the determination of profiles of resistance to insecticides. The altered enzymatic activity showed that the changes could have an important role in exposing resistance to insecticides. Therefore, monitoring enzymatic profiles in mosquito populations concomitantly to combating vectors is of fundamental importance.
Palavras-chave (keywords):
Aedes aegypti
Dengue Fever
Resistance mechanisms
Insecticides
NUNES, R.F.F., Oliveira, Jéssica Costa de, Souza, Marcos Antonio de, MARINHO, M.J.M, Grangeiro, Richardeson Fagner de Oliveira, Oliveira Pereira, Wogelsanger. Caracterização de perfis enzimáticos de cepas do Aedes aegypti do Estado do Rio Grande do Norte, Brasil. Cien Saude Colet [periódico na internet] (2015/set). [Citado em 24/12/2024].
Está disponível em: http://cienciaesaudecoletiva.com.br/artigos/caracterizacao-de-perfis-enzimaticos-de-cepas-do-aedes-aegypti-do-estado-do-rio-grande-do-norte-brasil/15315?id=15315&id=15315