Author:

• Carina Patussi - Patussi, C. - Joaçaba, SC - Universidade do Oeste de Santa Catarina (UNOESC), Joaçaba, Santa Catarina, Brasil - <caripatussi@yahoo.com.br>

Co-author(s):

• Márcia Bündchen - Bündchen, M. - Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Sul (IFRS), Porto Alegre, Rio Grande do Sul, Brasil. - <marcia.bundchen@poa.ifrs.edu.br>

Thematic Area:

Saúde e Ambiente

Abstract:

Tradescantia 4430 clone stamen hair mutation (Trad-SHM) bioassay was used to evaluate the genotoxicity of a herbicide comprising triazines (atrazine and simazine) by in situ exposure. Thirty plant vessels were exposed during the herbicide application (test group) and a control was maintained in a greenhouse (control group). Genotoxicity was expressed in terms of pink mutation events (EMPs) and the data analysis was performed by Student‘s t test comparing control and contaminated group after eight-days (C8D = 8 days control; T8D = 8 days test) and peak day (CPD = peak day control; TPD = peak day test). The exposure to the herbicide caused a significantly higher number of EMPs in the contaminated group (T8D = 2.27; TPD = 4.69) that of the control (C8D = 0.71; CPD = 0.62). This demonstrates that the Trad-SHM bioassay is sensitive and efficient and can be used as tool to assess the genotoxic potencial of environmental contaminants like triazines associated with adverse effects on human health.

Keywords:

pesticides genotoxity pink mutations environment human health

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